A versatile polypeptide platform for integrated recognition and reporting: affinity arrays for protein–ligand interaction analysis

…, GT Dolphin, B Liedberg, I Lundström…

Index: Enander, Karin; Dolphin, Gunnar T.; Liedberg, Bo; Lundstroem, Ingemar; Baltzer, Lars Chemistry - A European Journal, 2004 , vol. 10, # 10 p. 2375 - 2385

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Citation Number: 38

Abstract

Abstract A molecular platform for protein detection and quantification is reported in which recognition has been integrated with direct monitoring of target-protein binding. The platform is based on a versatile 42-residue helix–loop–helix polypeptide that dimerizes to form four- helix bundles and allows site-selective modification with recognition and reporter elements on the side chains of individually addressable lysine residues. The well-characterized ...