Experimental and kinetic studies of the Escherichia coli glucuronylsynthase: an engineered enzyme for the synthesis of glucuronide conjugates

SM Wilkinson, MA Watson, AC Willis…

Index: Wilkinson, Shane M.; Watson, Morgan A.; Willis, Anthony C.; McLeod, Malcolm D. Journal of Organic Chemistry, 2011 , vol. 76, # 7 p. 1992 - 2000

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Citation Number: 15

Abstract

The detection and study of glucuronide metabolites is essential in many fields including pharmaceutical development, sports drug testing, and the detection of agricultural residues. Therefore, the development of improved methods for the synthesis of glucuronide conjugates is an important aim. The glycosynthase derived from E. coli β-glucuronidase provides an efficient, scalable, single-step synthesis of β-glucuronides under mild ...