Bioorganic chemistry

Determination of the transglycosidation activity of NAD+ glycohydrolases with 4-(2′-alkyl-sulfanyl-vinyl)-pyridine derivatives generating chromophoric NAD+ analogs

K Pacaud, D Tritsch, A Burger, JF Biellmann

Index: Pacaud, Karine; Tritsch, Denis; Burger, Alain; Biellmann, Jean-Francois Bioorganic Chemistry, 2003 , vol. 31, # 4 p. 288 - 305

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Citation Number: 2

Abstract

The base exchange of nicotinamide with pyridine derivatives 1a–5a, catalyzed by pig brain NAD+ glycohydrolase and ADP-ribosyl cyclase from Aplysia californica, generated the corresponding NAD+ analogs 1b–5b. These analogs exhibited a high absorbance band in the visible region. The transglycosidation rate was determined by monitoring the absorbance increase. Among the tested derivatives,(E)-4-[2-(methylsulfanyl)-vinyl]- ...