New cyanine dyes as base surrogates in PNA: forced intercalation probes (FIT-probes) for homogeneous SNP detection
L Bethge, DV Jarikote, O Seitz
Index: Bethge, Lucas; Jarikote, Dilip Venkatrao; Seitz, Oliver Bioorganic and Medicinal Chemistry, 2008 , vol. 16, # 1 p. 114 - 125
Full Text: HTML
Citation Number: 75
Abstract
Forced intercalation probes (FIT-probes) are nucleic acid probes, in which an intercalator cyanine dye such as thiazole orange (TO) serves as a replacement of a canonical nucleobase. These probes signal hybridization by showing strong increases of fluorescence. TO in FIT-probes responds to adjacent base mismatches by attenuation of fluorescence intensities at conditions where both matched and mismatched target DNA ...
Related Articles:
[Walczyski, Krzysztof; Zuiderveld, Obbe P.; Timmerman, Henk European Journal of Medicinal Chemistry, 2005 , vol. 40, # 1 p. 15 - 23]
[Walczyski, Krzysztof; Zuiderveld, Obbe P.; Timmerman, Henk European Journal of Medicinal Chemistry, 2005 , vol. 40, # 1 p. 15 - 23]