Bioorganic & medicinal chemistry letters

Using peptidyl aldehydes in activity-based proteomics

E Sabidó, T Tarragó, E Giralt

Index: Sabido, Eduard; Tarrago, Teresa; Giralt, Ernest Bioorganic and Medicinal Chemistry Letters, 2009 , vol. 19, # 14 p. 3752 - 3755

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Citation Number: 5

Abstract

The broad inhibitory spectrum of aldehydes and the possibility that amino acid residues modulate their specificity point to the potential of using peptidyl aldehydes as activity-based probes. Here, we establish the potential of peptidyl aldehydes in activity-based proteomics by synthesizing different probes and using them to specifically label a well-known serine protease in an activity-dependent manner. From our results, peptidyl aldehydes emerge ...