Nonisotopic DNA detection system employing elastase and a fluorogenic rhodamine substrate

AF Johnson, MD Struthers, KB Pierson…

Index: Johnson, Arthur F.; Struthers, Mary D.; Pierson, Keith B.; Mangel, Walter F.; Smith, Lloyd M. Analytical Chemistry, 1993 , vol. 65, # 17 p. 2352 - 2359

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Citation Number: 20

Abstract

An alternative fluorescence-based method has been developed for the direct detection of small quantities of DNA in solution. In this system, a serine protease (elastase) is coupled to a DNA oligonucleotide through a disulfide linkage. A bis-(tetraa1anine)-derivatized rhodamine molecule (BZTAlaR) has been synthesized for use as a substrate. BZTAlaR is nonfluorescent in its derivatized form and shows negligible hydrolysis in solution. ...