Mapping the substrate selectivity of new hydrolases using colorimetric screening: lipases from Bacillus thermocatenulatus and Ophiostoma piliferum, esterases from …

…, GP Horsman, A Mezzetti, C Schmidt-Dannert…

Index: Liu, Andrew Man Fai; Somers, Neil A.; Kazlauskas, Romas J.; Brush, Terry S.; Zocher, Frank; Enzelberger, Markus M.; Bornscheuer, Uwe T.; Horsman, Geoff P.; Mezzetti, Alessandra; Schmidt-Dannert, Claudia; Schmid, Rolf D. Tetrahedron Asymmetry, 2001 , vol. 12, # 4 p. 545 - 556

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Citation Number: 85

Abstract

Recent advances in biochemistry and molecular biology have simplified the discovery and preparation of new hydrolases. Although these hydrolases might solve problems in organic synthesis, measuring their selectivity, especially enantioselectivity, remains tedious and time consuming. Recently, we developed a colorimetric screening method to measure the enantioselectivity of hydrolases. Here we apply this rapid screening method to map the ...