Helvetica chimica acta

Efficient Solid Phase Synthesis of Cleavable Oligodeoxynucleotides Based on a Novel Strategy for the Synthesis of 5′??S??(4, 4′??Dimethoxytrityl)??2′??deoxy??5′?? …

K Jahn??Hofmann, JW Engels

Index: Jahn-Hofmann, Kerstin; Engels, Joachim W. Helvetica Chimica Acta, 2004 , vol. 87, # 11 p. 2812 - 2828

Full Text: HTML

Citation Number: 9

Abstract

Abstract The incorporation of a specific cleavage site into an oligodeoxynucleotide can be achieved by utilizing the four 5′-S-(4, 4′-dimethoxytrityl)-2′-deoxy-5′-thionucleoside 3′-(2-cyanoethyl diisopropylphosphoramidites) 5 and 15a–c (Fig. 1). Based on the silver ion assisted cleavage of P [BOND] S and C [BOND] S bonds, we synthesized oligodeoxynucleotides with an achiral 5′-phosphorothioate linkage 3′–O–P–S–5′ by ...