Synthesis of lipid–oligonucleotide conjugates for RNA interference studies

S Grijalvo, SM Ocampo, JC Perales…

Index: Grijalvo, Santiago; Ocampo, Sandra M.; Perales, Jose C.; Eritja, Ramon Chemistry and Biodiversity, 2011 , vol. 8, # 2 p. 287 - 299

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Citation Number: 7

Abstract

Abstract The synthesis of RNA molecules carrying lipids at their 3′-termini and 5′-termini is reported. These conjugates were fully characterized by MALDI-TOF mass spectrometry and HPLC chromatography. The ability of these conjugates to silence gene expression was evaluated in the inhibition of the tumor necrosis factor. All the lipid [BOND] siRNA derivatives were compatible with RNA interference machinery if transfected with oligofectamine. In the ...