Dehydrogenation, oxidative denitration and ring contraction of N, N-dimethyl-5-nitrouracil by a Bacillus nitroreductase Nfr-A1

…, P Chaignon, D Sergent, S Dezard, J Ouazzani

Index: Cortial, Sylvie; Chaignon, Philippe; Sergent, Didier; Dezard, Sophie; Ouazzani, Jamal Journal of Molecular Catalysis B: Enzymatic, 2012 , vol. 76, p. 1 - 8

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Citation Number: 2

Abstract

Nfr-A1, a Bacillus subtilis nitroreductase, catalyzes the nitroreduction of a large panel of aromatic and heterocyclic nitro compounds, except those belonging to nitrouracil class of molecules. Besides nitroreduction, Nfr-A1 exhibits a strong NADH oxidase activity in the presence of oxygen, leading to high concentration of H2O2 (up to 200μM). In the presence of (N, N)-dimethyl-5-nitrouracil 1 (dim-NU), Nfr-A1 achieves the reduction of dim-NU ...