Detection of terminal mismatches on DNA duplexes with fluorescent oligonucleotides

U Asseline, M Chassignol, Y Aubert…

Index: Asseline, Ulysse; Chassignol, Marcel; Aubert, Yves; Roig, Victoria Organic and Biomolecular Chemistry, 2006 , vol. 4, # 10 p. 1949 - 1957

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Citation Number: 48

Abstract

This paper describes the design of terminal-mismatch discriminating fluorescent oligonucleotides (TMDFOs). The method is based on the use of sets of oligo-2′- deoxyribonucleotide probes linked via their 5′-ends, and varying-sized flexible polymethylene chains, to thiazole orange, with the linker being attached to the benzothiazole moiety. The sequence of each set of labelled probes was identical and ...