The Journal of Organic Chemistry

9-(2-Sulfo) fluorenylmethyloxycarbonyl chloride, a new reagent for the purification of synthetic peptides

RB Merrifield, AE Bach

Index: Merrifield,R.B.; Bach,A.E. Journal of Organic Chemistry, 1978 , vol. 43, p. 4808 - 4816

Full Text: HTML

Citation Number: 61

Abstract

A selective method was developed for the purification of synthetic peptides. It is based on the addition of the 9-(2-sulfo) fluorenylmethyloxycarbonyl (Sulfmoc) group to the free amine of the growing peptide chains at the end of a solid-phase synthesis. After cleavage from the support, the strongly acidic Sulfmoc-peptides are separated chromatographically from the nongrowing, terminated peptides. Finally, the Sulfmoc group is removed by mild base and ...