Fluorescence-based active site probes for profiling deubiquitinating enzymes

JF McGouran, HB Kramer, MM Mackeen…

Index: McGouran, Joanna F.; Kramer, Holger B.; MacKeen, Mukram M.; Di Gleria, Katalin; Altun, Mikael; Kessler, Benedikt M. Organic and Biomolecular Chemistry, 2012 , vol. 10, # 17 p. 3379 - 3383

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Citation Number: 8

Abstract

Novel ubiquitin-based active site probes including a fluorescent tag have been developed and evaluated. A new, functionalizable electrophilic trap is utilized allowing for late stage diversification of the probe. Attachment of fluorescent dyes allowed direct detection of endogenous deubiquitinating enzyme (DUB) activities in cell extracts by in-gel fluorescence imaging.