Substrate imprinted lipase nanogel for one-step synthesis of chloramphenicol palmitate
R Wang, Y Zhang, J Huang, D Lu, J Ge, Z Liu
Index: Wang, Rui; Zhang, Yifei; Huang, Jinhai; Lu, Diannan; Ge, Jun; Liu, Zheng Green Chemistry, 2013 , vol. 15, # 5 p. 1155 - 1158
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Citation Number: 26
Abstract
Enzymatic catalysis with high enantio-and regio-selectivity, which is attractive for green synthesis of chemicals, often suffers from low activity in organic solvents utilized as reaction media. Here, we describe a 'substrate-imprinted'lipase nanogel that displays high activity in organic solvents. The first step was to encapsulate lipase into polyacrylamide nanogel by an aqueous in situ polymerization. Then the lipase nanogel was lyophilized in the presence ...
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