Enhancement of bacterial mutagenicity of bifunctional alkylating agents by expression of mammalian glutathione S-transferase

…, SE Pemble, B Ketterer, FP Guengerich

Index: Thier, Ricarda; Mueller, Michael; Taylor, John B.; Pemble, Sally E.; Ketterer, Brian; Guengerich, F. Peter Chemical Research in Toxicology, 1995 , vol. 8, # 3 p. 465 - 472

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Citation Number: 58

Abstract

Recently, we inserted the plasmid vector pKK233-2 containing rat GSH S-transferase (GST) 5-5 cDNA into Salmonella typhimurium TA1535 and found that these bacteria [GST 5-5 (+) 1 expressed the protein and produced mutations when ethylene or methylene dihalides were added [Thier, R., Taylor, JB, Pemble, SE, Ketterer, B., Persmark, M., Humphreys, WG, and Guengerich, FP (1993) Proc. Natl. Acad. Sci. USA. 90, 8576-85801. After exposure to the ...