Engineered biosynthesis of nonribosomal lipopeptides with modified fatty acid side chains

…, B Wilkinson, CP Smith, J Micklefield

Index: Milne, Claire; Powell, Amanda; Jim, John; Al Nakeeb, Majid; Smith, Colin P.; Micklefield, Jason Journal of the American Chemical Society, 2006 , vol. 128, # 34 p. 11250 - 11259

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Citation Number: 32

Abstract

The biological properties of the calcium-dependent antibiotics (CDAs), daptomycin and related nonribosomal lipopeptides, depend to a large extent on the nature of the N-terminal fatty acid moiety. It is suggested that the chain length of the unusually short (C6) 2, 3- epoxyhexanoyl fatty acid moiety of CDA is determined by the specificity of the KAS-II enzyme encoded by fabF3 in the CDA biosynthetic gene cluster. Indeed, deletion of the ...