Specific and stable fluorescence labeling of histidine-tagged proteins for dissecting multi-protein complex formation

…, M Gavutis, R Tampé, J Piehler

Index: Lata, Suman; Gavutis, Martynas; Tampe, Robert; Piehler, Jacob Journal of the American Chemical Society, 2006 ,  vol. 128,  # 7  p. 2365 - 2372

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Citation Number: 168

Abstract

Labeling of proteins with fluorescent dyes offers powerful means for monitoring protein interactions in vitro and in live cells. Only a few techniques for noncovalent fluorescence labeling with well-defined localization of the attached dye are currently available. Here, we present an efficient method for site-specific and stable noncovalent fluorescence labeling of histidine-tagged proteins. Different fluorophores were conjugated to a chemical ...