A highly efficient method for site-specific modification of unprotected peptides after chemical synthesis

SJ Bark, S Schmid, KM Hahn

Index: Bark, Steven J.; Schmid, Sandra; Hahn, Klaus M. Journal of the American Chemical Society, 2000 , vol. 122, # 15 p. 3567 - 3573

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Citation Number: 32

Abstract

We have developed a highly efficient method for the site-specific attachment of biophysical probes to unprotected peptides after chemical synthesis. This methodology takes advantage of the selective reactivity of an N-methylaminooxy amino acid that is appropriately protected for direct incorporation during solid-phase peptide synthesis. The functional N- methylaminooxy group is unmasked using normal peptide cleavage conditions and is ...