Multiligand interactions at the combining site of anti-fluorescyl antibodies. Molecular recognition and connectivity

…, D Schloeder, A Tramontano

Index: Janjic; Schloeder; Tramontano Journal of the American Chemical Society, 1989 , vol. 111, # 16 p. 6374 - 6377

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Citation Number: 12

Abstract

Abstract: Partitioning of binding energy among the constituent subsites in a ligand-antibody complex can be useful in assessment of bimolecular processes in the combining site. Binding affinities of five anti-fluorescyl monoclonal antibodies for fluorescein (1) and two compounds representing its component parts, xanthenone (2) and benzoate, were studied by the fluorescence quenching technique. Dissociation constants were found to be in the ...