Photoactivable sphingosine as a tool to study membrane microenvironments in cultured cells

M Aureli, S Prioni, L Mauri, N Loberto, R Casellato…

Index: Aureli, Massimo; Prioni, Simona; Mauri, Laura; Loberto, Nicoletta; Casellato, Riccardo; Ciampa, Maria Grazia; Chigorno, Vanna; Prinetti, Alessandro; Sonnino, Sandro Journal of Lipid Research, 2010 , vol. 51, # 4 p. 798 - 808

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Citation Number: 4

Abstract

Abstract Human fibroblasts from normal subjects and Niemann-Pick A (NPA) disease patients were fed with two labeled metabolic precursors of sphingomyelin (SM),[3 H] choline and photoactivable sphingosine, that entered into the biosynthetic pathway allowing the synthesis of radioactive phosphatidylcholine and SM, and of radioactive and photoactivable SM ([3 H] SM-N 3). Detergent resistant membrane (DRM) fractions prepared from normal ...