Targeting the substrate binding site of E. coli nitrile reductase QueF by modeling, substrate and enzyme engineering

…, G Steinkellner, A Lyskowski, B Nidetzky…

Index: Wilding, Birgit; Winkler, Margit; Petschacher, Barbara; Kratzer, Regina; Egger, Sigrid; Steinkellner, Georg; Lyskowski, Andrzej; Nidetzky, Bernd; Gruber, Karl; Klempier, Norbert Chemistry - A European Journal, 2013 , vol. 19, # 22 p. 7007 - 7012

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Citation Number: 14

Abstract

Abstract Nitrile reductase QueF catalyzes the reduction of 2-amino-5-cyanopyrrolo [2, 3-d] pyrimidin-4-one (preQ 0) to 2-amino-5-aminomethylpyrrolo [2, 3-d] pyrimidin-4-one (preQ 1) in the biosynthetic pathway of the hypermodified nucleoside queuosine. It is the only enzyme known to catalyze a reduction of a nitrile to its corresponding primary amine and could therefore expand the toolbox of biocatalytic reactions of nitriles. To evaluate this ...