Phosphorus-31 nuclear magnetic resonance spectroscopic observation of the intracellular transformations of oncostatic cyclophosphamide metabolites
…, JD Robbins, W Egan, SM Ludeman
Index: Boyd, Victoria L.; Robbins, Joan D.; Egan, William; Ludeman, Susan M. Journal of Medicinal Chemistry, 1986 , vol. 29, # 7 p. 1206 - 1210
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Citation Number: 62
Abstract
Results Packed U937 human histiocytic lymphoma cellslg (0.5 mL; ca. 1.5 X lo8 cells) were incorporated into agarose gel threads, as described. 15-18 The cell-containing threads were perfused, in a recirculating fashion, 16-18 with Ham's F-12 medium (pH 7.4) supplemented with 10% of fetal calf serum. The 31P NMR spectra of the cells embedded in the gel threads were virtually identical with those obtained initially for dense cell suspensions (cell ...
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