Functional and conformational modulation of human cytochrome P450 1B1 by anionic phospholipids

HH Jang, DH Kim, T Ahn, CH Yun

Index: Jang, Hyun-Hee; Kim, Dong-Hyun; Ahn, Taeho; Yun, Chul-Ho Archives of Biochemistry and Biophysics, 2010 , vol. 493, # 2 p. 143 - 150

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Citation Number: 11

Abstract

We investigated the interaction of human P450 1B1 (CYP1B1) with various phospholipid bilayers using the N-terminally deleted (Δ2–4) CYP1B1 and (Δ2–26) CYP1B1 enzymes. Among anionic phospholipids, phosphatidic acid (PA) and cardiolipin specifically increased the catalytic activities, membrane binding affinities, and thermal stabilities of both CYP1B1 proteins when phosphatidylcholine matrix was gradually replaced with these anionic ...