Structural basis for the one-pot formation of the diarylheptanoid scaffold by curcuminoid synthase from Oryza sativa

H Morita, K Wanibuchi, H Nii, R Kato…

Index: Morita, Hiroyuki; Wanibuchi, Kiyofumi; Nii, Hirohiko; Kato, Ryohei; Sugio, Shigetoshi; Abe, Ikuro Proceedings of the National Academy of Sciences of the United States of America, 2010 , vol. 107, # 46 p. 19778 - 19783

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Citation Number: 26

Abstract

Abstract Curcuminoid synthase (CUS) from Oryza sativa is a plant-specific type III polyketide synthase (PKS) that catalyzes the remarkable one-pot formation of the C 6-C 7-C 6 diarylheptanoid scaffold of bisdemethoxycurcumin, by the condensation of two molecules of 4-coumaroyl-CoA and one molecule of malonyl-CoA. The crystal structure of O. sativa CUS was solved at 2.5-Å resolution, which revealed a unique, downward expanding active-site ...