Tetrahedron letters

Design and synthesis of an affinity probe that targets caspases in proteomic experiments

ML Liau, RC Panicker, SQ Yao

Index: Liau, Ming-Lee; Panicker, Resmi C.; Yao, Shao Q. Tetrahedron Letters, 2003 , vol. 44, # 5 p. 1043 - 1046

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Citation Number: 39

Abstract

The field of proteomics aims to study all proteins in the human proteome. This huge task may be accelerated by using active-site directed probes which profile proteins in an activity- dependent manner. Herein, we have developed a fluorescently-labeled affinity probe containing chemical reactivity specific towards caspases. Preliminary assays and proof-of- concept experiments demonstrated that this probe exhibits strong chemical reactivity ...