Journal of Peptide Science

The use of 2, 2′??dithiobis (5??nitropyridine)(DTNP) for deprotection and diselenide formation in protected selenocysteine??containing peptides

AL Schroll, RJ Hondal, S Flemer

Index: Schroll, Alayne L.; Hondal, Robert J.; Flemer, Stevenson Journal of Peptide Science, 2012 , vol. 18, # 3 p. 155 - 162

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Citation Number: 9

Abstract

Selenocysteine (Sec, U) is the '21st'proteinogenic amino acid because it shares three key properties with the other 20 common amino acids: it has its own codon (UGA), it has its own unique tRNA, and it is co-translationally inserted at the ribosome [1, 2]. Heterologous expression and production of selenoproteins is difficult because of the complexity of the UGA recoding machinery needed by the ribosome to translate UGA as a sense codon for ...