Fluorescent substrates for potential use in enzyme-linked immunosorbent assay of membrane-bound nucleic acids

S Fujita, M Momiyama, Y Kondo, N Kagiyama…

Index: Fujita, Satoshi; Momiyama, Masayoshi; Kondo, Yasumitsu; Kagiyama, Naoto; Hori, Samuel H.; Toru, Takeshi Analytical Chemistry, 1994 , vol. 66, # 8 p. 1347 - 1353

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Citation Number: 16

Abstract

A number of phosphorylated fluorochromes were synthesized and tested for the alkaline phosphatase-linked fluorescence assay of membrane-bound nucleic acids. 3-Hydroxy-N-2'- biphenyl-2-naphthalenecarboxamide phosphate ester (HBNP) was found to be the most suitable for the assay. Nonfluorescent HBNP is hydrolyzed by phospholipase bound to the probe DNA to the highly fluorescent 3-hydroxy-N, 2'-biphenyl-2-naphtha-lenecarboxamide ...