Fast cleavage and deprotection of oligonucleotides
MP Reddy, NB Hanna, F Farooqui
Index: Reddy, M. P.; Hanna, N. B.; Farooqui, Firdous Tetrahedron Letters, 1994 , vol. 35, # 25 p. 4311 - 4314
Full Text: HTML
Citation Number: 126
Abstract
Abstract We have developed methylamine/ammonia as a fast cleavage and deprotection reagent which effects complete cleavage of oligonucleotides from the solid support in 5 min at room temperature and complete deprotection in 5 min at 65 C. The problem of transamination side product formation, faced with the commonly used dC bz (10.0% side product) upon treatment with methylamine/ammonia, has been successfully solved by the ...
Related Articles:
Synthesis and cytostatic evaluation of 4-N-alkanoyl and 4-N-alkyl gemcitabine analogues
[Kraszewski; Delort; Teoule Tetrahedron Letters, 1986 , vol. 27, # 7 p. 861 - 864]
Engineering tethered DNA molecules by the convertible nucleoside approach
[MacMillan, Andrew M.; Verdine, Gregory L. Tetrahedron, 1991 , vol. 47, # 14/15 p. 2603 - 2616]
[Saladino, Raffaele; Mincione, Enrico; Crestini, Claudia; Mezzetti, Maurizio Tetrahedron, 1996 , vol. 52, # 19 p. 6759 - 6780]
Engineering tethered DNA molecules by the convertible nucleoside approach
[MacMillan, Andrew M.; Verdine, Gregory L. Tetrahedron, 1991 , vol. 47, # 14/15 p. 2603 - 2616]
Engineering tethered DNA molecules by the convertible nucleoside approach
[MacMillan, Andrew M.; Verdine, Gregory L. Tetrahedron, 1991 , vol. 47, # 14/15 p. 2603 - 2616]