Thienylpyridines as a New Fluorescent Reagent. I. Determination of Primary Alkylamines with 5-(4-Pyridyl)-2-thiophenecarbaldehyde Using HPLC

R Nakajima, A Yamamoto, T Hara

Index: Nakajima, Riichiro; Yamamoto, Akifumi; Hara, Tadashi Bulletin of the Chemical Society of Japan, 1990 , vol. 63, # 7 p. 1968 - 1972

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Citation Number: 7

Abstract

5-(4-Pyridyl)-2-thiophenecarbaldehyde has been prepared as a new fluorescent derivatization reagent and utilized for the determination of primary alkylamines. A definite detection limit was achieved for the amines by using a method based on the combination of precolumn derivatization and postcolumn hydrolysis–detection of the original fluorescent reagent regenerated from the derivatives. Approximately a 0.1 pmol (10 μl injection)(S/N= ...