Immunoassay of unconjugated estriol in serum of pregnant women monitored by chemiluminescence

W Klingler, O Haupt, G Postel, R Knuppen

Index: Klingler; Haupt; Postel; Knuppen Steroids, 1983 , vol. 42, # 2 p. 123 - 136

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Citation Number: 11

Abstract

Abstract 6-Oxoestriol-6-(0-carboxymethyl) oxime-aminobutylethyl-isoluminol conjugate was synthesized. This luminogenic estriol derivative enabled us to develop a solid phase immunoassay method for the determination of unconjugated estriol in serum of pregnant women by the measurement of the bound estriol-isoluminol conjugate upon oxidation with a hydrogen peroxide/microperoxidase system. The sensitivity of the assay was 700 pmol/1. ...