Highly Efficient Chemoenzymatic Synthesis of Methyl (R)??o??Chloromandelate, a Key Intermediate for Clopidogrel, via Asymmetric Reduction with Recombinant …

T Ema, S Ide, N Okita, T Sakai

Index: Ema, Tadashi; Ide, Sayaka; Okita, Nobuyasu; Sakai, Takashi Advanced Synthesis and Catalysis, 2008 , vol. 350, # 13 p. 2039 - 2044

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Citation Number: 73

Abstract

Abstract Methyl (R)-o-chloromandelate [(R)-1], which is an intermediate for a platelet aggregation inhibitor named clopidogrel, was obtained in> 99% ee by the asymmetric reduction of methyl o-chlorobenzoylformate (2) with recombinant Escherichia coli overproducing a versatile carbonyl reductase. A remarkable temperature effect on productivity was observed in the whole-cell reduction of 2, and the optimum productivity as ...