Mechanism of the enzymic elimination of ammonia from 3-substituted aspartic acids by 3-methylaspartase

NP Botting, M Akhtar, MA Cohen, D Gani

Index: Botting, Nigel P.; Akhtar, Mahmoud; Cohen, Mark A.; Gani, David Journal of the Chemical Society, Chemical Communications, 1987 , # 18 p. 1371 - 1373

Full Text: HTML

Citation Number: 10

Abstract

Kinetic experiments with 3-methylaspartase, using aspartic, 3-methylaspartic, and 3- ethylaspartic acid and the appropriate C-3 deuteriated isotopomers as substrates, reveal that C (3)–H bond cleavage is partially rate-limiting for 3-methylaspartic acid, much less rate- limiting for 3-ethylaspartic acid, and not rate-