Synthesis of 2-acetamido-2-deoxy-3-O-β-D-galactopyrano-syl-D-galactose by the sequential use of β-D-galactosidases from bovine testes and Escherichia coli

L Hedbys, E Johansson, K Mosbach, PO Larsson…

Index: Hedbys, Lars; Johansson, Elisabet; Mosbach, Klaus; Larsson, Per-Olof; Gunnarsson, Alf; Svensson, Sigfrid Carbohydrate Research, 1989 , vol. 186, p. 217 - 224

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Citation Number: 62

Abstract

Abstract β-d-Galp-(1→ 3)-d-GalNAc (1) was synthesised from lactose and GalNAc on a mmolar scale by transgalactosylation using β-d-galactosidase from bovine testes. The large proportions of unwanted oligosaccharides in the product mixture were removed by treatment with β-d-galactosidase from E. coli, which left 1, monosaccharides, and a small proportion of trisaccharides. Carbon-Celite chromatography then gave 1 in a yield of 21% based on the ...