Steroids

Development of a new sensitive and specific time-resolved fluoroimmunoassay (TR-FIA) of chlormadinone acetate in the serum of treated menopausal women

J Fiet, F Giton, J Auzerie, H Galons

Index: Fiet, Jean; Giton, Frank; Auzerie, Jack; Galons, Herve Steroids, 2002 , vol. 67, # 13-14 p. 1045 - 1055

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Citation Number: 6

Abstract

We describe the development of a serum chlormadinone acetate (CMA) time-resolved fluoroimmunoassay (TR-FIA). We prepared haptens (3-CMO-chlormadinone acetate and 6- chloropregna-4, 6-dien-17, 20-diol-3-one-20-hemisuccinate), biotinylated tracers (3 ( biotinylaminopropylamido) 3-CMO-chlormadinone acetate and 3-(6-chloropregna-4, 6-dien- 17, 20-diol-3-one-20-hemisuccinylamino) 1-biotinylaminopropane), and immunogens ...