Enzymatic synthesis of activated esters and their subsequent use in enzyme-based peptide synthesis

…, DTS Rijkers, RMJ Liskamp, PJLM Quaedflieg

Index: Nuijens, Timo; Cusan, Claudia; Schepers, Annette C.H.M.; Kruijtzer, John A.W.; Rijkers, Dirk T.S.; Liskamp, Rob M.J.; Quaedflieg, Peter J.L.M. Journal of Molecular Catalysis B: Enzymatic, 2011 , vol. 71, # 1-2 p. 79 - 84

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Citation Number: 25

Abstract

Abstract Chemoenzymatic peptide synthesis is potentially the most cost-efficient technology for the synthesis of short and medium-sized peptides. However, there are still some limitations when challenging peptides, eg containing sterically demanding acyl donors, non- proteinogenic amino acids or proline residues, are to be synthesized. To remedy these limitations, special ester moieties have been used that are specifically recognized by the ...