Chemoenzymatic synthesis of glutamic acid analogues: substrate specificity and synthetic applications of branched chain aminotransferase from Escherichia coli

M Xian, S Alaux, E Sagot, T Gefflaut

Index: Xian, Mo; Alaux, Sebastien; Sagot, Emmanuelle; Gefflaut, Thierry Journal of Organic Chemistry, 2007 , vol. 72, # 20 p. 7560 - 7566

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Citation Number: 26

Abstract

A new route to α-keto acids is described, based on the ozonolysis of enol acetates obtained from α-substituted β-keto esters. Escherichia coli branched chain aminotransferase (BCAT) activity toward a variety of substituted 2-oxoglutaric acids was demonstrated analytically. BCAT was shown to have a broad substrate spectrum, complementary to that of aspartate aminotransferase, and to offer access to a variety of glutamic acid analogues. The ...