Plasmid??based, D??aminopeptidase??catalysed synthesis of (R)??amino acids

…, K Kishino, A Yamada, S Hanamoto…

Index: Asano, Yasuhisa; Kishino, Katsuhiko; Yamada, Akiko; Hanamoto, Sawako; Kondo, Kiyosi Recueil des Travaux Chimiques des Pays-Bas, 1991 , vol. 110, # 5 p. 206 - 208

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Citation Number: 20

Abstract

Abstract The gene for D-aminopeptidase from Ochrobactrum anthropi SCRC Cl-38 was cloned in Escherichia coli JM109. An expression plasmid pCl38DP (4.5 kb) was constructed. The amount of the enzyme in a cell-free extract of E. coli JM109/pCl38DP was elevated up to 288000 units/liter culture, which is about 3600-fold over that of O. anthropi SCRC Cl-38. It was calculated that the enzyme comprised about 30% of the total extractable cellular ...