Enzymatic Synthesis of Cytidine 5′-Monophospho-N-acetylneuraminic Acid
T Hamamoto, S Takeda, T Noguchi
Index: Hamamoto, Tomoki; Takeda, So; Noguchi, Toshitada Bioscience, Biotechnology and Biochemistry, 2005 , vol. 69, # 10 p. 1944 - 1950
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Citation Number: 5
Abstract
We have established an efficient method for enzymatic production of cytidine 5′- monophospho-N-acetylneuraminic acid (CMP-NeuAc) from inexpensive materials, N- acetylglucosamine (GlcNAc) and cytidine 5′-monophosphate (CMP). The Haemophilus influenzae nanE gene encoding GlcNAc 6-phosphate (GlcNAc 6-P) 2-epimerase and the Campylobacter jejuni neuB1 gene encoding N-acetylneuraminic acid (NeuAc) synthetase, ...
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