Empirical rules for the enantiopreference of lipase from Aspergillus niger toward secondary alcohols and carboxylic acids, especially α-amino acids

LE Janes, RJ Kazlauskas

Index: Janes, Lana E.; Kazlauskas, Romas J. Tetrahedron Asymmetry, 1997 , vol. 8, # 22 p. 3719 - 3733

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Citation Number: 31

Abstract

Lipase from Aspergillus niger (ANL, Amano lipase AP) catalyzes enantioselective hydrolysis and acylation reactions. To aid in the design of new applications of this lipase, we propose two empirical rules that predict which enantiomer reacts faster. For secondary alcohols, a rule proposed previously for other lipases also works for ANL, but with lower reliability (77%, 37 of 48 examples). For carboxylic acids, we examined both crude and partially-purified ...