Peptide segment synthesis catalyzed by the semisynthetic enzyme thiolsubtilisin
T Nakatsuka, T Sasaki, ET Kaiser
Index: Nakatsuka, T.; Sasaki, T.; Kaiser, E. T. Journal of the American Chemical Society, 1987 , vol. 109, # 12 p. 3808 - 3810
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Citation Number: 174
Abstract
PA A I-AA,-ON+ HOC, H, CI-p (“, I a P= bcnzylovycarbonyl (Z) or tluorcnylmethoxycarbonyl (FMOC) group, AA,= N-tcrniinal mino acid or peptide component. and AA,= C-terminal amino acid or pcptidc component. the coupling of the segments, the low solubility of side- chainprotected peptides in the organic solvents employed, and low yields. For such reasons, protease-catalyzed peptide synthesis5 has drawn considerable attention because most of ...
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