Enzymatic Kolbe–Schmitt reaction to form salicylic acid from phenol: enzymatic characterization and gene identification of a novel enzyme, Trichosporon moniliiforme …

K Kirimura, H Gunji, R Wakayama, T Hattori…

Index: Biochemical and Biophysical Research Communications, , vol. 394, # 2 p. 279 - 284

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Citation Number: 23

Abstract

... Biochemical and Biophysical Research Communications. ... activity, the following standard reaction conditions were used: the mixture contained 100 μg of protein, 20 mM phenol, and 2.5 M KHCO 3 in 100 mM K 2 HPO 4 –KH 2 PO 4 buffer (pH 7.0) with a final volume of 1 ml ...