Structure–activity analysis of base and enzyme-catalyzed 4-hydroxybenzoyl coenzyme A hydrolysis

F Song, Z Zhuang, D Dunaway-Mariano

Index: Song, Feng; Zhuang, Zhihao; Dunaway-Mariano, Debra Bioorganic Chemistry, 2007 , vol. 35, # 1 p. 1 - 10

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Citation Number: 10

Abstract

In this study, the second-order rate constant k2 of base-catalyzed hydrolysis and the values of kcat, Km and kcat/Km of wild-type Pseudomonas sp. CBS3 4-hydroxybenzoyl coenzyme A (4-HBA-CoA) thioesterase-catalyzed hydrolysis of 4-HBA-CoA and its para-substituted analogs were measured. For the base-catalyzed hydrolysis, the plot of logk2 vs the σ value of the para-substituents was linear with a slope (ρ) of 1.5. In the case of the enzyme- ...