An assay for human telomeric G-quadruplex DNA binding drugs

D Watkins, N Ranjan, S Kumar, C Gong…

Index: Watkins, Derrick; Ranjan, Nihar; Kumar, Sunil; Gong, Changjun; Arya, Dev P. Bioorganic and Medicinal Chemistry Letters, 2013 , vol. 23, # 24 p. 6695 - 6699

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Citation Number: 3

Abstract

Abstract Compounds that stabilize the G-quadruplexes formed by human telomeres can inhibit the telomerase activity and are potential cancer therapies. We have developed an assay for the screening of compounds with high affinity for human telomeric G-quadruplexes (HTG). The assay uses a thiazole orange fluorescent reporter molecule conjugated to the aminoglycoside, neomycin, as a probe in a fluorescence displacement assay. The ...