Tetrahedron: Asymmetry

Universal chromogenic substrates for lipases and esterases

J Grognux, D Wahler, E Nyfeler, JL Reymond

Index: Grognux, Johann; Wahler, Denis; Nyfeler, Erich; Reymond, Jean-Louis Tetrahedron Asymmetry, 2004 , vol. 15, # 18 p. 2981 - 2989

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Citation Number: 35

Abstract

Chromogenic mono-and diacyl-glycerol analogues were prepared and tested as substrates against lipases and esterases in a microtiter plate setup. Thirty-three enzyme samples were analyzed for activity at 100, 10, and 1μgmL− 1. The reaction rates observed were visualized by RGB-color coding of activities, and analyzed by cluster analysis. The C8-, C10-, and C12- monoesters of the nitrophenol-derived diol 13 reacted very strongly across all enzyme ...