Native RNA-Sequencing Throws its Hat into the Transcriptomics Ring

Shobbir Hussain

Index: 10.1016/j.tibs.2018.02.007

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Abstract

De novo sequence-level surveys of transcriptomes have previously relied on sequencing via a DNA intermediate. While such methods can yield massive data sets, various problems mean that these do not always accurately reflect the true innate composition of transcriptomes. Enter Garalde et al., who present for the first time highly parallel native RNA-Sequencing (RNA-seq), with potentially disruptive future-implications for the transcriptomics field.