Resveratrol reverses the adverse effects of diet-induced obese murine model on oocyte quality and zona pellucida softening

Zhenzhen Jia, Zeyang Feng, Lining Wang, Hao Li, Hongyu Wang, Dingqi Xu, Xin Zhao, Dao-Fu Feng, Xi-Zeng Feng

Index: 10.1039/C8FO00149A

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Abstract

Reproductive dysfunction associated with obesity is becoming increasingly among women of reproductive age, such as infertility and increasing risk for miscarriage. In females, reproductive disorders is linked to declined quality of oocytes. Using the model of diet-induced obesity we have investigated the possible effects of obesity on oocyte quality, including metabolism, lipid accumulation, ROS levels, meiosis and the change of spindle structure in Metaphase II (MII). Our study showed that obesity induced by high fat diet could impair oocyte meiosis, destroy spindle assembly, and promote oxidative stress and abnormal mitochondrial distributions. With the addition of resveratrol, the negative impact of diet-induced obesity on the quality of oocytes was alleviated to some extent. In addition, we also found that obesity makes mouse oocytes soften, and resveratrol can restore the Zona pellucida of oocytes to the control group. In conclusion, resveratrol can reverse the adverse effects of obesity on oocyte, which is beneficial to subsequent embryonic development.