Simultaneous determination of Multi-Residue and Multi-Class antibiotics in aquaculture shrimps by UPLC-MS/MS

Sushil Kumar Saxena, Rajesh Rangasamy, Anoop A Krishnan, Dhirendra P Singh, Sumedh P. Uke, Praveen Kumar Malekadi, Anoop S Sengar, D Peer Mohamed, Ananda Gupta

Index: 10.1016/j.foodchem.2018.04.018

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Abstract

An accurate, reliable and fast multi-residue, multi-class method using ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) was developed and validated for simultaneous determination and quantification of 24 pharmacologically active substances of three different classes (Quinolones including fluoroquinolones, sulphonamides and tetracylines) in aquaculture shrimps. Sample preparation involves extraction with acetonitrile containing 0.1% formic acid and followed by clean up with n-hexane and 0.1% methanol in water by UPLC-MS/MS within 8 min. The method was validated according to European Commission Decision 2002/657. Acceptable values were obtained for linearity (5 to 200μg kg−1), specificity, Limit of Quantification (5 to 10 μg kg−1), recovery (between 83 and 100%), repeatability (RSD < 9%), within lab reproducibility (RSD<15%), reproducibility (RSD≤22%), decision limit (105 to 116 μg kg−1) and detection capability (110 to 132 μg kg−1). The validated method was applied to aquaculture shrimp samples from India.