Interior surface modification of bacteriophage MS2

JM Hooker, EW Kovacs, MB Francis

Index: Hooker, Jacob M.; Kovacs, Ernest W.; Francis, Matthew B. Journal of the American Chemical Society, 2004 , vol. 126, # 12 p. 3718 - 3719

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Citation Number: 250

Abstract

An efficient strategy for the interior surface functionalization of MS2 viral capsids is reported, featuring a new hetero-Diels-Alder bioconjugation reaction. After virus isolation, the RNA genome was removed from the spherical particles by exposure to pH 11.8 conditions for a period of 4 h. Following this, 180 tyrosine residues on the interior surface of each “empty” capsid shell were modified by using a site-selective diazonium-coupling reaction. To ...