Analytical biochemistry

Antibody-free peptide substrate screening of serine/threonine kinase (protein kinase A) with a biotinylated detection probe

M Kim, YS Park, DS Shin, J Kim, BG Kim, YS Lee

Index: Kim, Mira; Park, Yong-Sun; Shin, Dong-Sik; Kim, Jaehi; Kim, Byung-Gee; Lee, Yoon-Sik Analytical Biochemistry, 2011 , vol. 413, # 1 p. 30 - 35

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Citation Number: 2

Abstract

Being different from anti-phosphotyrosine antibodies, anti-phosphoserine-or anti- phosphothreonine-specific antibodies with high affinity for the detection of serine/threonine kinase substrates are not readily available. Therefore, chemical modification methods were developed for the detection of phosphoserine or threonine in the screening of protein kinase substrates based on β-elimination and Michael addition. We have developed a biotin- ...