Mapping the substrate selectivity and enantioselectivity of esterases from thermophiles
NA Somers, RJ Kazlauskas
Index: Somers, Neil A.; Kazlauskas, Romas J. Tetrahedron Asymmetry, 2004 , vol. 15, # 18 p. 2991 - 3004
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Citation Number: 19
Abstract
To identify potential applications of nineteen esterases from thermophiles, we mapped their substrate selectivity and enantioselectivity using a library of 50 esters. We measured the selectivities colorimetrically using Quick E, which uses pH indicators to detect hydrolysis and a chromogenic reference compound as an internal control. The substrate selectivity mapping revealed one esterase, E018b, with a strong preference for acetyl esters (14-to ...
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